信号通路筛选?LabEx抗体芯片实验服务
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信号通路筛选?”芯“有灵犀一点通

 

找到信号通路的关键节点是复杂且耗时耗力的,抗体芯片试剂盒可以在一个实验室里监测信号通路中众多关键信号组分的变化,为您节省宝贵的时间和试剂!

 

每个试剂盒有2张玻片,每张玻片上有8或16张垫片,最多可同时检测32个样本,这样可使研究者在一次实验里有充分的自由度来检测多种浓度,细胞系或时间节点的样本!

 

抗体芯片基于夹心法ELISA原理进行设计,将众多信号通路关键节点蛋白的捕获抗体布于样品孔内做成抗体点阵,随后加入相应的识别抗体混合物,再利用两种不同的显色方式呈现结果,即化学发光法和荧光法检测!

 

目前的抗体芯片产品列表如下:

 

  Products 荧光法   化学发光法
  PathScan® Akt Signaling Antibody Array Kit #9700 #9474
  PathScan® EGFR Signaling Antibody Array Kit #12785 #12622
  PathScan® Intracellular Signaling Array Kit #7744 #7323
  PathScan® RTK Signaling Antibody Array Kit #7949 #7982
  PathScan® Stress and Apoptosis Signaling Antibody Array Kit(NEW) #12923 #12856
  PathScan® Th1/Th2/Th17 Cytokine Antibody Array Kit #13124 #13047
  PathScan® Immune Cell Signaling Antibody Array Kit(NEW) #13788 #13792
  PathScan® Cancer Phenotype Antibody Array Kit #14822 #14821

 

自己不方便?我们来帮您!LabEx接受抗体芯片实验服务啦!

 

【实验室化学发光法检测仪器】

 

【实验室做出的结果照片及灰度值分析】

 

 注:全国服务都可接受,我们设有样本全国干冰运送冷链!

 

PathScan Akt Signaling Antibody Array Kit
#9700(荧光法)& #9474(化学发光法)
32次检测,16个蛋白靶点,512个数据点

 

 

Akt Signaling

 

  Target Phosphorylation Site
1 Positive Control N/A
2 Negative Control N/A
3 Akt Thr308
4 Akt Ser473
5 S6 Ribosomal Protein Ser235/236
6 AMPKa Thr172
7 PRAS40 Thr246
8 mTOR Ser2481
9 GSK-3a Ser21
10 GSK-3b Ser9
11 p70 S6 Kinase Thr389
12 p70 S6 Kinase Thr421/Ser424
13 Bad Ser112
14 RSK1 Thr421/Ser424
15 PTEN Ser380
16 PDK 1 Ser241
17 Erk1/2 Thr202/Tyr204
18 4E-BP1 Thr37/46

 

PathScan EGFR Signaling Antibody Array Kit
#12785(荧光法)& #12622(化学发光法)
16次检测,24个蛋白靶点,384个数据点

 

EGFR Signaling

  Target Phosphorylation Site
1 Positive Control N/A
2 Negative Control N/A
3 EGFR Total
4 EGFR Thr669
5 EGFR Tyr845
6 EGFR Tyr998
7 EGFR Tyr1068
8 EGFR - L858R Mutant Total
9 EGFR - E746-A750 Deletion Total†
10 MEK1 Total
11 MEK2 Total
12 MEK1/2 Ser221
13 MEK1/2 Ser217/221
14 Stat3 Tyr705
15 HER2/ErbB2 Total
16 HER2/ErbB2 Tyr1196
17 HER2/ErbB2 Tyr1221/1222
18 Met Total
19 Met Tyr1349
20 Met Tyr1234/1235
21 PLCÝ1 Total
22 PLCÝ1 Ser1248
23 Akt Thr308
24 Akt Ser473
25 Erk1/2 Thr202/Tyr204
26 PARP Asp214 (Cleavage)

 

PathScan Intracellular Signaling Antibody Array Kit
#7744(荧光法)& #7323(化学发光法)
32次检测,18个蛋白靶点,576个数据点

 

Intracellular Signaling

 

  Target Phosphorylation Site Modification
1 Positive Control N/A N/A
2 Negative Control N/A N/A
3 ERK1/2 Thr202/Tyr204 Phosphorylation
4 Stat1 Tyr701 Phosphorylation
5 Stat3 Tyr705 Phosphorylation
6 Akt Thr308 Phosphorylation
7 Akt Ser473 Phosphorylation
8 AMPKa Thr172 Phosphorylation
9 S6 Ribosomal Protein Ser235/236 Phosphorylation
10 mTOR Ser2448 Phosphorylation
11 HSP27 Ser78 Phosphorylation
12 Bad Ser112 Phosphorylation
13 p70 S6 Kinase Thr389 Phosphorylation
14 PRAS40 Thr246 Phosphorylation
15 p53 Ser15 Phosphorylation
16 p38 Thr180/Tyr182 Phosphorylation
17 SAPK/JNK Thr183/Tyr185 Phosphorylation
18 PARP Asp214 Cleavage
19 Caspase-3 Asp175 Cleavage
20 GSK-3b Ser9 Phosphorylation

 

PathScan RTK Signaling Antibody Array Kit
#7949(荧光法)& #7982(化学发光法)
16次检测,39个蛋白靶点,624个数据点

 

Receptor Tyrosine Kinases

 

  Target Phosphorylation Site Family
1 EGFR/ErbB1 pan-Tyr EGFR
2 HER2/ErbB2 pan-Tyr EGFR
3 HER3/ErbB3 pan-Tyr EGFR
4 FGFR1 pan-Tyr FGFR
5 FGFR3 pan-Tyr FGFR
6 FGFR4 pan-Tyr FGFR
7 InsR pan-Tyr Insulin R
8 IGF-IR pan-Tyr Insulin R
9 TrkA/NTRK1 pan-Tyr NGFR
10 TrkA/NTRK2 pan-Tyr NGFR
11 Met/HGFR pan-Tyr HGFR
12 Ron/MST1R pan-Tyr HGFR
13 Ret pan-Tyr Ret
14 ALK pan-Tyr LTK
15 PDGFR pan-Tyr PDGFR
16 c-Kit/SCFR pan-Tyr PDGFR
17 FLT3/Flk2 pan-Tyr PDGFR
18 M-CSFR/CSF-1R pan-Tyr PDGFR
19 EphA1 pan-Tyr EphR
20 EphA2 pan-Tyr EphR
21 EphA3 pan-Tyr EphR
22 EphB1 pan-Tyr EphR
23 EphB3 pan-Tyr EphR
24 EphB4 pan-Tyr EphR
25 Tyro3/Dtk pan-Tyr Axl
26 Axl pan-Tyr Axl
27 Tie2/TEK pan-Tyr Tie
28 VEGFR2/KDR pan-Tyr VEGFR

 

Signaling Nodes

 

  Target Phosphorylation Site Family
29 Akt/PKB/Rac Thr308 Akt
30 Akt/PKB/Rac Ser473 Akt
31 p44/42 MAPK (ERK1/2) p44/42 MAPK (ERK1/2) MAPK
32 S6 Ribosomal Protein Ser235/236 RSK
33 c-Abl pan-Tyr Abl
34 IRS-1 pan-Tyr IRS
35 Zap-70 pan-Tyr Zap-70
36 Src pan-Tyr Src
37 Lck pan-Tyr Src
38 Stat1 pan-Tyr Stat
39 Stat3 pan-Tyr Stat

 

PathScan® Stress and Apoptosis Signaling Antibody Array Kit
#12923(荧光法)#12856(化学发光法)
32次检测,19个蛋白八点,608个数据点

 

Stress Apoptosis Signaling

  Target Site Modification
1 Positive Control N/A N/A
2 Negative Control N/A N/A
3 P44/42 MAPK(ERK1/2) Thr202/Tyr204 Phosphorylation
4 Akt Ser473 Phosphorylation
5 Bad Ser136 Phosphorylation
6 HSP27 Ser82 Phosphorylation
7 Smad2 Ser465/467 Phosphorylation
8 p53 Ser15 Phosphorylation
9 p38 MAPK Thr180/ Thr182 Phosphorylation
10 SAPK/JNK Thr183/Tyr185 Phosphorylation
11 PARP Asp214 Cleavage
12 Caspase-3 Asp175 Cleavage
13 Caspase-7 Asp198 Cleavage
14 IkBa Total N/A
15 Chk1 Ser345 Phosphorylation
16 Chk2 Thr68 Phosphorylation
17 IkBa Ser32/36 Phosphorylation
18 elF2a Ser51 Phosphorylation
19 TAK1 Ser412 Phosphorylation
20 Survivin<, /TD> Total N/A
21 a-Tubulin Total N/A

 

PathScanTh1/Th2/Th17 Cytokine Antibody Array Kit
#13124(荧光法)& #13047(化学发光法)
32次检测,12个蛋白靶点,384个数据点

 

Cytokine Targets

 

  Target
1   Positive Control
2   Negative Control
3   IL-2
4   IL-4
5   IL-5
6   IL-6
7   IL-8
8   IL-10
9   IL-12 p70
10   IL-13
11   IL-17A
12   TNF-a
13   GM-CSF
14   IFN-g

 

 

PathScan Immune Cell Signaling Antibody Array Kit
#13788(荧光法)& #13792(化学发光法)
32次检测,19个蛋白靶点,608个数据点

 

Immune Cell Signaling

 

  Target Site Modification
1 Positive Control N/A N/A
2 Negative Control N/A N/A
3 p44/42 MAPK (Erk1/2) Thr202/Tyr204 Phosphorylation
4 Akt Ser473 Phosphorylation
5 p38 MAPK Thr180/Tyr182 Phosphorylation
6 SAPK/JNK Thr183/Tyr185 Phosphorylation
7 Caspase-7 Asp198 Cleavage
8 Ikba Total N/A
9 Ikba Ser32/36 Phosphorylation
10 TAK1 Ser412 Phosphorylation
11 Stat1 Tyr701 Phosphorylation
12 Stat1 Ser727 Phosphorylation
13 Stat3 Tyr705 Phosphorylation
14 Stat3 Ser727 Phosphorylation
15 Stat5 Tyr694 Phosphorylation
16 Stat6 Tyr641 Phosphorylation
17 Lck Tyr416 Phosphorylation
18 Syk Tyr352 Phosphorylation
19 Zap-70 Tyr319 Phosphorylation
20 Rig-I Total N/A
21 IRF-3 Total N/A

 

PathScan® Cancer Phenotype Antibody Array Kit
#14822(荧光法)#14821(化学发光法)
32次检测,19个蛋白八点,608个数据点

 

Cancer Phenotype

 

  Target Site Modification
1 Positive Control N/A N/A
2 Negative Control N/A N/A
3 CD31(PECAM-1) Total N/A
4 EpCAM Total N/A
5 Vimanim Total N/A
6 CD44 Total N/A
7 CD45 Total N/A
8 PCNA Total N/A
9 Ki-67 Total N/A
10 p27 Kip1 Total N/A
11 E-Cadhenin Total N/A
12 N-Cadhenin Total N/A
13 VE-Dadherin Total N/A
14 MUC1 Total N/A
15 Rb Ser807/811 Phosphorylation
16 HF-1a Total N/A
17 Survivin Total N/A
18 pS3 Total N/A
19 HER2/Erb82 Total N/A
20 Mat Total N/A
21 EGF Recoptc Total N/A

 

 

 

详见LabEx网站( http://www.u-labex.com)或来电咨询!
基因水平:PCR Array
蛋白水平:MSD、Luminex、CBA、Antibody Array、ELISA
细胞水平:磁珠分选、流式细胞分析
组织水平:多重免疫组化、病理分析

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