DSP人空间蛋白组图谱_570蛋白_36
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15 Lipoxygenase 1,15-PGDH,2B4,5 Lipoxygenase/5-LO,ACE2,Actin,active YAP1,Adenosine Receptor A2a,AIF,AKR1B10,AKT1 (phospho S473),AKT1 (phospho T450),AKT3 + AKT2 + AKT1,ALDH1A1,Aldolase,Aldolase B+Aldolase C,ALK,alpha smooth muscle Actin,AMPK alpha 1,Androgen Receptor,APC,APG5L/ATG5,Argonaute-2,ARID1A,ARID3A,ASS1,ATF2,ATF3,ATG12,ATG7,ATM,ATM (phospho S1981),ATR (phospho S428),Aurora A,Axl,Bad,Bak,Bax,Bcl-2,Bcl6,Bcl-XL,Beclin 1,beta 2 Microglobulin,beta Actin,beta Arrestin 1,beta Catenin,beta glucuronidase (GUSB),beta Tubulin,BFL-1/GRS,Bid,Bim,BRAF,BRCA1,Brd4,BRG1,BTK,C Reactive Protein,C4a,C5 / C5b,Carbonic Anhydrase 3/CA3,Carbonic Anhydrase 9/CA9,Caspase-10/CASP-10,Caspase-3,Caspase-3 p12,Catalase,Cathepsin G,Cathepsin K,Cathepsin S,Caveolin-1,CCL18,CCR3,CCR5,CCR6,CCR7,CD103,CD11a,CD11b,CD11c,CD127,CD133,CD134 / OX40L receptor,CD14,CD15,CD16,CD163,CD177,CD18,CD20,CD22,CD27,CD272/BTLA,CD276,CD28,CD3 epsilon,CD31,CD33,CD34,CD36,CD38,CD39,CD3D,CD3G,CD4,CD40,CD40 Ligand,CD44,CD44v6,CD45,CD45RA,CD47,CD63,CD64,CD66b,CD68,CD70,CD73,CD74,CD79a,CD79b,CD8 alpha,CD83,CD86,CD90 / Thy1,CD94,CD99,CDK1,CDK1 (phospho T161) + CDK2 / CDK3 (phospho T160),CDK1 + Cdk2 + Cdk3 (phospho T14),CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15),Cdk2,Cdk4,Cdk6,CDKN2A/p14ARF,CDKN2A/p16INK4a,c-Fos,Chk1,Chk2,cIAP2,c-Jun,c-Jun (phospho S63),c-Kit,Cleaved PARP1,c-Maf,c-Myb (phospho S11),c-Myc,c-Myc (phospho S62),Collagen I,Collagen IV,COX2 / Cyclooxygenase 2,CPT1A,CREB,CREB (phospho S133),CREBBP,CSF-1-R,CTLA4,Cullin 1/CUL-1,CXCL10/IP-10,CXCL5 + CXCL6,CXCR3,CXCR4,CXCR5,CXCR6,Cyclin A2,Cyclin B1,Cyclin D1,Cyclin E1,Cyclin E2,Cyclophilin A,Cytokeratin 1,Cytokeratin 14,Cytokeratin 17,Cytokeratin 19,Cytokeratin 5,Cytokeratin 8,Cytokeratin 8 (phospho S431),Dihydrofolate reductase (DHFR),Dnmt1,DR5,DUSP6,E Cadherin,EGFR,EGFR (phospho Y1068),EMR1/ADGRE1,ENO1 + ENO2 + ENO3,eNOS,EpCAM,ErbB2 / HER2,ErbB2 / HER2 (phospho Y877),ErbB3 / HER3,ErbB4 / HER4 (phospho Y1162),ErbB4 / HER4+ErbB2 / HER2,ERG,ERK1,ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187),ERK1 (phospho T202) + ERK2 (phospho T185),ERK2,Estrogen Receptor alpha,ETS1,FABP4,FADD,FAK,FANCI,Fas,Fatty Acid Synthase,FBP2,FGF2,FGFR2,Fibronectin,Fos B,FOXO1A,FOXO4/AFX,FOXP3,Galectin 3,gamma H2A.X (phospho S139),GATA3,GGT1/GGT,GITR,GLB1/Beta-galactosidase,GLP-1,GLP-1R,Glucocorticoid Receptor (phospho S226),Glucose 6 Phosphate Dehydrogenase,Glucose Transporter GLUT1,Glutaminase,Glutathione Peroxidase 4,GNLY/Granulysin,Granzyme A,Granzyme B,Granzyme K,GRP78 BiP,GSK3 beta,GSK3 beta (phospho S9),HDAC1,HDAC2,HDAC3,HDAC4 + 5 + 9,HDAC6,HDAC9,Heme Oxygenase 1,Hexokinase 1,Hexokinase II,HIF-1 alpha,Histone H2A (acetyl K5),Histone H2A (phospho T120),Histone H2A.X,Histone H2B (acetyl K5),Histone H3 (acetyl K14),Histone H3 (acetyl K18),Histone H3 (acetyl K27),Histone H3 (acetyl K9),Histone H3 (di methyl K4),Histone H3 (di methyl K79),Histone H3 (mono methyl K4),Histone H3 (phospho S10),Histone H3 (phospho S28),Histone H3 (tri methyl K27),Histone H3 (tri methyl K36),Histone H3 (tri methyl K9),HLA A,HLA B7,HLA E,HLA F,HLA G,HLA-C,HLA-DPB1,HLA-DR,HMGB1,HMGB2,Hsp27,Hsp27 (phospho S78),Hsp70,Hsp90,HuR / ELAVL1,ICAM1,ICOS,IDH1,IDH2,IFIT1,IFNGR1,IGF1 Receptor,IGF2BP1/IMP1,Ikaros,IKB alpha,IKK alpha,IKK beta,IKK gamma/NEMO,IKKi/IKKe,IL-1 alpha,IL-1 beta,IL-12A,IL-12RB1,IL-15RA,IL-18,IL-1RA,IL-2 Receptor alpha,IL22 RA2/IL-22BP,IL-22RA1,IL-2RG,IL-33,IL3RA/CD123,IL4,IL-5RA,IL-6R,IMP3,Indoleamine 2, 3-dioxygenase,iNOS,Insulin,Insulin Receptor alpha,Insulin Receptor beta,Integrin alpha 5,Integrin alpha 6,Integrin beta 1,Interferon gamma,IRF3,IRF4,IRF5,ISG15,JAK3,JNK1 + JNK2 + JNK3 (phospho T183+T183+T221),JunD,KDM3A / JHDM2A,Ki67,KIR2DL1 + KIR2DL2,KIR2DL3,KIR3DL1 + KIR3DS1,KMT6 / EZH2,KMT6/EZH2 (phospho T487),Lactate Dehydrogenase,LAG-3,Lamin A + Lamin B1 + Lamin C,LAMP1,LAMP2A,LC3B,Lck,LDL Receptor,LEF1,LILRB3,Liver Arginase,LYVE1,Macrophage Inflammatory Protein 1 alpha / CCL3,Macrophage Inflammatory Protein 3 alpha,Mannose Receptor,M-CSF,MDM2 (phospho S166),MEK1,MEK1 (phospho S298),MEK2,MEK4/MKK4 (phospho S80),MEKK3,MelanA,Melanoma gp100,Met (c-Met),Met (c-Met) (phospho Y1349),MICA + MICB,MLH1,MMP1,MMP14,MMP3,MMP7,MMP8,MMP9,Monoamine Oxidase A/MAO-A,MRP8,MS4A2,MSH2,MSH6,mTOR,mTOR (phospho S2448),MUC1,Mucin 5AC,Musashi 1 / Msi1,MX1,MyD88,Myeloperoxidase,N Cadherin,NAK/TBK1,Nanog,NCAM1,NCR1,Nectin 2,Nectin-4,Neutrophil Elastase,NFAT2,NFkB p105 / p50,NF-kB p65,NKG2A + NKG2E + NKG2C,NKG2D,Notch1,NQO1,NRF1,NSD3,Occludin,Oct-2,Oct4,Osteopontin,p27 KIP 1,p27 KIP 1 (phospho S10),p38 (phospho T180),p38 beta/MAPK11 + p38 alpha/MAPK14,p40 - DeltaNp63,p53,p53 (acetyl K373),p53 (phospho S392),p53 (phospho S46),p57 Kip2,p63,p73,PAK1,PAK1+PAK2+PAK3 (phospho S141 + S144 + S154),pan Cytokeratin,Pan Trk,PARP1,PCNA,PD1,PDGF B,PDGFR alpha,PDGFR alpha + PDGFR beta,PD-L1,PD-L2,Periostin,PF4,PI 3 Kinase catalytic subunit alpha/PIK3CA,PI 3 Kinase catalytic subunit gamma/PI3K-gamma,PI 3 Kinase p85 alpha,PI 3 Kinase p85 beta,PKC beta 1,PLCG 2,Podoplanin / gp36,Poliovirus Receptor/PVR,PP2A alpha + beta,PRAME,PRAS40,PRDM1/Blimp1,Pro Caspase-8,Progesterone Receptor,Progesterone Receptor (phospho S190),Prostate Specific Antigen,PSMA,PTCH2,PTEN,PTEN (phospho T366),PTGES2/Gbf1,PU.1/Spi1,Rad51,RAGE,Raptor,Ras,Rb,Rb (phospho S608),Rb (phospho S807),Rb (phospho T252),Rb (phospho T356),Rb (phospho T373),Rb (phospho T780),Rel B,Ret,Retinoic Acid Receptor beta,Rho,Rho A + B + C,ROCK1,ROCK2 + ROCK1,ROS1,RPS6 (phospho S235 + S236),RPS6 (phospho S240 + S244),RSK1 p90,RSK1 p90 (phospho T359),RUNX1 / AML1 + RUNX3 + RUNX2,S100 beta,S100A12/CGRP,S100A4,S100A8 + S100A9,S100A9,S100P,S6K1,Scavenging Receptor SR-BI,SCF,SDHA,SFRP1,SFRP4,SHP1,SHP2,SIGLEC5,SIRT1,SIRT1 (phospho S47),SIRT2,SIRT3,SLC7A5/LAT1,Smac/Diablo,SMAD1 + SMAD5 + SMAD9 (phospho S463 + S465 + S467),Smad2,Smad2 (phospho S255),Smad2 (phospho S467),Smad3,Smad3 (phospho S423 + S425),Smad4,SMAD5,SOCS3,SOD2/MnSOD,SOD2/MnSOD (acetyl K68),Somatostatin Receptor 2,Sonic Hedgehog,SOX9,SQSTM1 / p62,Src,ST2,STAT1,STAT1 (phospho S727),STAT2,STAT3,STAT3 (phospho Y705),STAT5 (phospho Y694),STAT5a,STAT5b,STAT6,STAT6 (phospho Y641),STING,Sumo 1,Survivin,Syk,Syk (phospho Y323),Syk (phospho Y352) +ZAP70 (phospho Y319),Syndecan-1,TAK1,TAP2,T-bet / Tbx21,TBR2 / Eomes,TEF1/TEAD-1,TGF beta 1,Thymidylate Synthase,TIM 3,Tissue Factor,TLR7,TLR8,TLR9,TMPRSS2,TMS1/ASC,TNF alpha,TNFAIP3,Topoisomerase I,Topoisomerase II alpha,Topoisomerase II alpha (phospho S1106),Topoisomerase II alpha (phospho T1343),TRAF1,TRAF2,TRAF6,Transferrin Receptor,TROP2,Tuberin,Tuberin (phospho S1254),Tuberin (phospho T1462),UBE2C,Ubiquitin,uPA Receptor/U-PAR,Urokinase,VCAM1,VEGF Receptor 1,VEGF Receptor 2,VEGFA,VEGFD,Vimentin,VISTA,Von Willebrand Factor,Wee1,Wee1 (phospho S642),Wilms Tumor Protein,Wnt3a,YAP1,YAP1 (phospho S127),YKL-40/CHI3L1,ZAP70,ZEB1
服务货号:
LXDSPH36-IPA
交付周期:
需咨询
价格:
¥171,034.00/芯片,36个AOI
提交意向
收藏
服务货号:
LXDSPH36-IPA
交付周期:
需咨询
价格:
¥171,034.00/芯片,36个AOI
提交意向
收藏
  • 产品介绍
  • 技术平台介绍

产品介绍

检测指标: 点击检测指标名称可查看该指标具体功能介绍
15 Lipoxygenase 115-PGDH2B45 Lipoxygenase/5-LOACE2Actinactive YAP1Adenosine Receptor A2aAIFAKR1B10AKT1 (phospho S473)AKT1 (phospho T450)AKT3 + AKT2 + AKT1ALDH1A1AldolaseAldolase B+Aldolase CALKalpha smooth muscle ActinAMPK alpha 1Androgen ReceptorAPCAPG5L/ATG5Argonaute-2ARID1AARID3AASS1ATF2ATF3ATG12ATG7ATMATM (phospho S1981)ATR (phospho S428)Aurora AAxlBadBakBaxBcl-2Bcl6Bcl-XLBeclin 1beta 2 Microglobulinbeta Actinbeta Arrestin 1beta Cateninbeta glucuronidase (GUSB)beta TubulinBFL-1/GRSBidBimBRAFBRCA1Brd4BRG1BTKC Reactive ProteinC4aC5 / C5bCarbonic Anhydrase 3/CA3Carbonic Anhydrase 9/CA9Caspase-10/CASP-10Caspase-3Caspase-3 p12CatalaseCathepsin GCathepsin KCathepsin SCaveolin-1CCL18CCR3CCR5CCR6CCR7CD103CD11aCD11bCD11cCD127CD133CD134 / OX40L receptorCD14CD15CD16CD163CD177CD18CD20CD22CD27CD272/BTLACD276CD28CD3 epsilonCD31CD33CD34CD36CD38CD39CD3DCD3GCD4CD40CD40 LigandCD44CD44v6CD45CD45RACD47CD63CD64CD66bCD68CD70CD73CD74CD79aCD79bCD8 alphaCD83CD86CD90 / Thy1CD94CD99CDK1CDK1 (phospho T161) + CDK2 / CDK3 (phospho T160)CDK1 + Cdk2 + Cdk3 (phospho T14)CDK1 + CDK2 + CDK3 + CDK5 (phospho Y15)Cdk2Cdk4Cdk6CDKN2A/p14ARFCDKN2A/p16INK4ac-FosChk1Chk2cIAP2c-Junc-Jun (phospho S63)c-KitCleaved PARP1c-Mafc-Myb (phospho S11)c-Mycc-Myc (phospho S62)Collagen ICollagen IVCOX2 / Cyclooxygenase 2CPT1ACREBCREB (phospho S133)CREBBPCSF-1-RCTLA4Cullin 1/CUL-1CXCL10/IP-10CXCL5 + CXCL6CXCR3CXCR4CXCR5CXCR6Cyclin A2Cyclin B1Cyclin D1Cyclin E1Cyclin E2Cyclophilin ACytokeratin 1Cytokeratin 14Cytokeratin 17Cytokeratin 19Cytokeratin 5Cytokeratin 8Cytokeratin 8 (phospho S431)Dihydrofolate reductase (DHFR)Dnmt1DR5DUSP6E CadherinEGFREGFR (phospho Y1068)EMR1/ADGRE1ENO1 + ENO2 + ENO3eNOSEpCAMErbB2 / HER2ErbB2 / HER2 (phospho Y877)ErbB3 / HER3ErbB4 / HER4 (phospho Y1162)ErbB4 / HER4+ErbB2 / HER2ERGERK1ERK1 (phospho T202 + Y204) + ERK2 (phospho T185 + Y187)ERK1 (phospho T202) + ERK2 (phospho T185)ERK2Estrogen Receptor alphaETS1FABP4FADDFAKFANCIFasFatty Acid SynthaseFBP2FGF2FGFR2FibronectinFos BFOXO1AFOXO4/AFXFOXP3Galectin 3gamma H2A.X (phospho S139)GATA3GGT1/GGTGITRGLB1/Beta-galactosidaseGLP-1GLP-1RGlucocorticoid Receptor (phospho S226)Glucose 6 Phosphate DehydrogenaseGlucose Transporter GLUT1GlutaminaseGlutathione Peroxidase 4GNLY/GranulysinGranzyme AGranzyme BGranzyme KGRP78 BiPGSK3 betaGSK3 beta (phospho S9)HDAC1HDAC2HDAC3HDAC4 + 5 + 9HDAC6HDAC9Heme Oxygenase 1Hexokinase 1Hexokinase IIHIF-1 alphaHistone H2A (acetyl K5)Histone H2A (phospho T120)Histone H2A.XHistone H2B (acetyl K5)Histone H3 (acetyl K14)Histone H3 (acetyl K18)Histone H3 (acetyl K27)Histone H3 (acetyl K9)Histone H3 (di methyl K4)Histone H3 (di methyl K79)Histone H3 (mono methyl K4)Histone H3 (phospho S10)Histone H3 (phospho S28)Histone H3 (tri methyl K27)Histone H3 (tri methyl K36)Histone H3 (tri methyl K9)HLA AHLA B7HLA EHLA FHLA GHLA-CHLA-DPB1HLA-DRHMGB1HMGB2Hsp27Hsp27 (phospho S78)Hsp70Hsp90HuR / ELAVL1ICAM1ICOSIDH1IDH2IFIT1IFNGR1IGF1 ReceptorIGF2BP1/IMP1IkarosIKB alphaIKK alphaIKK betaIKK gamma/NEMOIKKi/IKKeIL-1 alphaIL-1 betaIL-12AIL-12RB1IL-15RAIL-18IL-1RAIL-2 Receptor alphaIL22 RA2/IL-22BPIL-22RA1IL-2RGIL-33IL3RA/CD123IL4IL-5RAIL-6RIMP3Indoleamine 2 3-dioxygenaseiNOSInsulinInsulin Receptor alphaInsulin Receptor betaIntegrin alpha 5Integrin alpha 6Integrin beta 1Interferon gammaIRF3IRF4IRF5ISG15JAK3JNK1 + JNK2 + JNK3 (phospho T183+T183+T221)JunDKDM3A / JHDM2AKi67KIR2DL1 + KIR2DL2KIR2DL3KIR3DL1 + KIR3DS1KMT6 / EZH2KMT6/EZH2 (phospho T487)Lactate DehydrogenaseLAG-3Lamin A + Lamin B1 + Lamin CLAMP1LAMP2ALC3BLckLDL ReceptorLEF1LILRB3Liver ArginaseLYVE1Macrophage Inflammatory Protein 1 alpha / CCL3Macrophage Inflammatory Protein 3 alphaMannose ReceptorM-CSFMDM2 (phospho S166)MEK1MEK1 (phospho S298)MEK2MEK4/MKK4 (phospho S80)MEKK3MelanAMelanoma gp100Met (c-Met)Met (c-Met) (phospho Y1349)MICA + MICBMLH1MMP1MMP14MMP3MMP7MMP8MMP9Monoamine Oxidase A/MAO-AMRP8MS4A2MSH2MSH6mTORmTOR (phospho S2448)MUC1Mucin 5ACMusashi 1 / Msi1MX1MyD88MyeloperoxidaseN CadherinNAK/TBK1NanogNCAM1NCR1Nectin 2Nectin-4Neutrophil ElastaseNFAT2NFkB p105 / p50NF-kB p65NKG2A + NKG2E + NKG2CNKG2DNotch1NQO1NRF1NSD3OccludinOct-2Oct4Osteopontinp27 KIP 1p27 KIP 1 (phospho S10)p38 (phospho T180)p38 beta/MAPK11 + p38 alpha/MAPK14p40 - DeltaNp63p53p53 (acetyl K373)p53 (phospho S392)p53 (phospho S46)p57 Kip2p63p73PAK1PAK1+PAK2+PAK3 (phospho S141 + S144 + S154)pan CytokeratinPan TrkPARP1PCNAPD1PDGF BPDGFR alphaPDGFR alpha + PDGFR betaPD-L1PD-L2PeriostinPF4PI 3 Kinase catalytic subunit alpha/PIK3CAPI 3 Kinase catalytic subunit gamma/PI3K-gammaPI 3 Kinase p85 alphaPI 3 Kinase p85 betaPKC beta 1PLCG 2Podoplanin / gp36Poliovirus Receptor/PVRPP2A alpha + betaPRAMEPRAS40PRDM1/Blimp1Pro Caspase-8Progesterone ReceptorProgesterone Receptor (phospho S190)Prostate Specific AntigenPSMAPTCH2PTENPTEN (phospho T366)PTGES2/Gbf1PU.1/Spi1Rad51RAGERaptorRasRbRb (phospho S608)Rb (phospho S807)Rb (phospho T252)Rb (phospho T356)Rb (phospho T373)Rb (phospho T780)Rel BRetRetinoic Acid Receptor betaRhoRho A + B + CROCK1ROCK2 + ROCK1ROS1RPS6 (phospho S235 + S236)RPS6 (phospho S240 + S244)RSK1 p90RSK1 p90 (phospho T359)RUNX1 / AML1 + RUNX3 + RUNX2S100 betaS100A12/CGRPS100A4S100A8 + S100A9S100A9S100PS6K1Scavenging Receptor SR-BISCFSDHASFRP1SFRP4SHP1SHP2SIGLEC5SIRT1SIRT1 (phospho S47)SIRT2SIRT3SLC7A5/LAT1Smac/DiabloSMAD1 + SMAD5 + SMAD9 (phospho S463 + S465 + S467)Smad2Smad2 (phospho S255)Smad2 (phospho S467)Smad3Smad3 (phospho S423 + S425)Smad4SMAD5SOCS3SOD2/MnSODSOD2/MnSOD (acetyl K68)Somatostatin Receptor 2Sonic HedgehogSOX9SQSTM1 / p62SrcST2STAT1STAT1 (phospho S727)STAT2STAT3STAT3 (phospho Y705)STAT5 (phospho Y694)STAT5aSTAT5bSTAT6STAT6 (phospho Y641)STINGSumo 1SurvivinSykSyk (phospho Y323)Syk (phospho Y352) +ZAP70 (phospho Y319)Syndecan-1TAK1TAP2T-bet / Tbx21TBR2 / EomesTEF1/TEAD-1TGF beta 1Thymidylate SynthaseTIM 3Tissue FactorTLR7TLR8TLR9TMPRSS2TMS1/ASCTNF alphaTNFAIP3Topoisomerase ITopoisomerase II alphaTopoisomerase II alpha (phospho S1106)Topoisomerase II alpha (phospho T1343)TRAF1TRAF2TRAF6Transferrin ReceptorTROP2TuberinTuberin (phospho S1254)Tuberin (phospho T1462)UBE2CUbiquitinuPA Receptor/U-PARUrokinaseVCAM1VEGF Receptor 1VEGF Receptor 2VEGFAVEGFDVimentinVISTAVon Willebrand FactorWee1Wee1 (phospho S642)Wilms Tumor ProteinWnt3aYAP1YAP1 (phospho S127)YKL-40/CHI3L1ZAP70ZEB1

技术平台介绍

近年来,随着生物技术和分析方法的不断进步,定量蛋白质和基因水平的研究在理解疾病机制和寻找治疗靶点等领域扮演着至关重要的角色。此外,深入了解组织生物学也需要考虑到空间维度的因素,因此空间分析在该领域的发展备受关注。Nature Methods杂志将空间多组学技术列为2022年最令人期待的七项技术之一,目前已有多家企业推出了空间多组学技术的商业化产品,有助于研究人员探索空间转录组的奥秘。

Nanostring GeoMx DSP技术提供结合了组织影像分析技术和高通量分子定量技术,能够在组织切片原位实现多靶标蛋白和全转录组的空间原位表达谱分析。通过捕获具有形态学背景的图像,按照组织形态、表型、单个细胞、按网格或者绘制区域轮廓,研究者可以选择感兴趣的区域进行分析。

DSP 空间多组学实验全流程

1、标记荧光抗体与待测指标共孵育:一张5μm厚度的切片同时用成像试剂(荧光标记的抗体/探针)和定量试剂(Oligo标记抗体/探针)混合杂交

2、ROI区域圈选:可以选择任何区域,任何形状和大小的ROI

3、区域划分&紫外光解DSP Barcode:每个圈选的目标区域依次被紫外光照射,并切断定量试剂上偶联的Oligo序列。在不接触样本的情况下,用微毛细管快速吸出解离的Oligo序列,使样本切片完整。

4、逐个ROI收集DSP Barcode96孔板: Oligo标签被收集到96孔收集板中,每一个孔对应组织切片上选择的一个ROI/AOI

5、Barcode计算、测序和数据分析:收集的Oligo利用高通量测序(NGS)或 在NanoStringnCounter平台上进行定量。

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