Non-small cell lung cancer-derived exosomes promote proliferation, phagocytosis, and secretion of microglia via exosomal microRNA in the metastatic microenvironment

Brain metastases; Exosomes; Microglia; Non–small cell lung cancer; miR1246.
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Peng Chen, Ying Li, Rui Liu, Yi Xie, Yu Jin, Minghuan Wang, Zhiyuan Yu, Wei Wang, Xiang Luo

  • Transl Oncol
  • 5
  • 2023 Jan;27:101594.
  • Human
  • 抗体芯片
  • 呼吸系统
  • 呼吸系统
  • 其它细胞
  • 肺癌
  • C5a,IL-4,IL-27,CD40 Ligand,IL-5,IL-32 alpha,G-CSF,IL-6,CXCL10/IP-10,GM-CSF,IL-8,CXCL11/I-TAC,CXCL1/GRO alpha,IL-10,CCL2/MCP-1,CCL1/I-309,IL-12 p70,MIF,ICAM-1,IL-13,MIP-1 alpha/MIP-1 beta,IFN-gamma,IL-16,CCL5/RANTES,IL-1 alpha,IL-17,CXCL12/SDF-1,IL-1 beta,IL-17E,Serpin E1/PAI-1,IL-1ra,IL-18,TNF-alpha,IL-2,IL-21,TREM-1

相关货号

LXAH036-2

Abstract

Non-small cell lung cancer (NSCLC) is the most common tumor that metastasizes to the brain. It is now accepted that the successful colonization and growth of tumor cells are determined by the interaction between tumor cells and the tumor microenvironment (TME). Microglia, brain innate immune cells, have been reported to play a vital role in the establishment of brain metastases. As essential mediators of intercellular communications, tumor-derived exosomes have an important role in the pathogenesis and progression of cancer by transferring their cargos to specific recipient cells. The crosstalk between microglia and tumor-derived exosomes has been extensively described. However, it is still unclear whether metastatic NSCLC cells secret exosomes to microglia and regulate the microglial functions. Here, our results showed that microglia aggregated in the brain metastatic sites. Meanwhile, microglia could take up the exosomes derived from NSCLC cells, leading to alterations of microglial morphology and increased proliferation, phagocytosis, and release of inflammatory cytokines including interleukin-6, interleukin-8, and CXCL1. Further investigation indicated that miR1246 was the most enriched microRNA in NSCLC-derived exosomes and mediated the partial effects of exosomes on microglia. Notably, miR1246 was also upregulated in the plasmatic exosomes of NSCLC patients. These results offer a new insight into the impact of NSCLC-derived exosomes on microglia and provide a new potential biomarker for diagnosing NSCLC.Keywords:Brain metastases; Exosomes; Microglia; Non–small cell lung cancer; miR1246.
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