In vitro production of immunoregulatory cytokines (IFN-α, IL-31, TNF-β, IL-17A, IL-7, IL-1RA, IL-1α, IL-10, IL-15, IL-21, IL-22, IL-23, IL-27, and IL-9) by dendritic cell cultures was compared in ski athletes and healthy donors. Effect of prolonged intense physical exercise on secretory activity of immune cells was investigated. In both groups, secretion of IL-1RA, IL-10, IL-1α by dendritic cells was revealed, but there were significant differences in IL-1RA, IL-1α content (p<0.05) with lower level in the group of athletes. Production of IL-17A and IL-7 by dendritic cells in the group of athletes was not detected. In athletes, several proinflammatory cytokines (IFN-α, IL-31, and TNF-β) were secreted by cells in high concentrations, in contrast to the control group. In both groups, dendritic cells did not secrete IL-15, IL-21, IL-22, IL-23, IL-27, and IL-9.