Antibody therapy is one of the fastest growing areas within the biopharmaceutical field. Immunological properties and biological activity are two of the most critical elements of a therapeutic antibody. The development and validation of analytical approaches provide an appropriate means for antibody immunological characterization, while the emergence of new technologies and advanced biological research and discovery help attain a better understanding of the mechanism of action of antibodies, and therefore facilitate the development of new therapeutic antibodies. This review describes the characteristics of therapeutic antibodies from both immunological and biological perspectives. Recent developments and current implementation of related technologies are discussed.