Here, we outline detailed protocols to isolate and profile murine splenic dendritic cells (DCs) through advanced flow cytometry of the myeloid compartment and single-cell transcriptomic profiling with integrated cell surface protein expression through CITE-seq. This protocol provides a general transferrable road map for different tissues and species. For complete details on the use and execution of this protocol, please refer to Lukowski et al. (2021).
Keywords:Cell isolation; Flow Cytometry/Mass Cytometry; Health Sciences; High Throughput Screening; Immunology; Molecular Biology; Sequence analysis; Sequencing; Single Cell.