Cell surface protein profiling is a generic tool to determine the quality of cultured mammalian cell lines. Flow cytometry is commonly employed as the screening technique of choice, but it consumes a large quantity of cells. We have evaluated an electrochemiluminescent (ECL)-based platform, the Meso Scale Discovery (MSD) Sector 6000 Imager, for cell surface protein detection with fewer than 100 cells per well. The detection of CD13 on human foreskin fibroblast (HFF) cells was evaluated using indirect detection of the reverse-phase immunoassays in 96-well and 384-well plate formats. The study has shown robust cell surface detection of CD13 on HFF cells using the 384-well plate format, with a LOD of 15.3 ± 6.5 cells (n=3). The 96-well plate format exhibited far greater variability between experiments, such that the best LOD obtained was 42.5 cells per well. We have demonstrated, with our model system, the feasibility to perform cell-binding assays with as few as 15 cells per well of a 384-well microplate using the MSD platform.

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乐备实（上海优宁维生物科技股份有限公司旗下全资子公司），是国内专注于提供高质量蛋白检测以及组学分析服务的实验服务专家，自2018年成立以来，乐备实不断寻求突破，公司的服务技术平台已扩展到单细胞测序、空间多组学、流式检测、超敏电化学发光、Luminex多因子检测、抗体芯片、PCR Array、ELISA、Elispot、PLA蛋白互作、多色免疫组化、DSP空间多组学等30多个，建立起了一套涵盖基因、蛋白、细胞以及组织水平实验的完整检测体系。

 
我们可提供从样本运输、储存管理、样本制备、样本检测到检测数据分析的全流程服务。凭借严格的实验室管理流程、标准化实验室操作、原始数据储存体系以及实验项目管理系统，已经为超过3000家客户单位提供服务，年检测样本超过100万，受到了广大客户的信任与支持。