Purpose: Dynamic remodeling of the extracellular matrix (ECM) around tumor cells is crucial for the tumor progressions. However, the mechanism is not well defined. Here, we aimed to reveal the underlying mechanism of ECM induced metastasis and provide innovative strategy to suppress the distant metastasis induced by ECM. Materials and methods: IHC was used to detect the expression of target proteins. H&E staining was used to evaluate the growth of tumor in vivo. Using wound healing and transwell assay, we examined the ability of cell to metastasis. We employed IF and Western blot to detect the expression of target proteins. And qRT-PCR was used to examine the target genes in mRNA level. We also applied flow cytometry to examine the percent of CD133+ cell population. Results: Herein, we observed elevated expression of type I collagen in colorectal cancer tissues from patients with high metastasis. Additionally, colorectal cancer cells cultured on 2D collagen reveal obviously enhanced capability of metastasis and tumorigenesis both in vitro and in vivo. We demonstrated that the activation of PI3K/AKT signal induced by integrin α2β1 resulted in the enhanced metastatic capability and stemness of colorectal cancer cells. Moreover, we found that Snail worked as the downstream of PI3K/AKT signaling, resulting in the intensive invasion and metastasis of colorectal cancer. Blocking the pathway by applying E7820 successfully reversed the type I collagen induced distant metastasis in colorectal cancer. Conclusion: Combining E7820 and chemotherapeutic agents to block the integrin α2β1/PI3K/AKT/Snail signaling pathway revealed dramatic enhanced tumor suppression and provided an innovative approach for clinical colorectal cancer treatment.Keywords: Colorectal cancer; EMT; PI3K/AKT pathway; Snail; Type I collagen.

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乐备实（上海优宁维生物科技股份有限公司旗下全资子公司），是国内专注于提供高质量蛋白检测以及组学分析服务的实验服务专家，自2018年成立以来，乐备实不断寻求突破，公司的服务技术平台已扩展到单细胞测序、空间多组学、流式检测、超敏电化学发光、Luminex多因子检测、抗体芯片、PCR Array、ELISA、Elispot、PLA蛋白互作、多色免疫组化、DSP空间多组学等30多个，建立起了一套涵盖基因、蛋白、细胞以及组织水平实验的完整检测体系。

 
我们可提供从样本运输、储存管理、样本制备、样本检测到检测数据分析的全流程服务。凭借严格的实验室管理流程、标准化实验室操作、原始数据储存体系以及实验项目管理系统，已经为超过3000家客户单位提供服务，年检测样本超过100万，受到了广大客户的信任与支持。