Fatigue is a persistent and debilitating symptom following radiation therapy for prostate cancer. However, it is not well-understood how radiation targeted to a small region of the body can lead to broad changes in behavior. In this study, we used targeted pelvic irradiation of healthy male mice to test whether inflammatory signaling mediates changes in voluntary physical activity levels. First, we tested the relationship between radiation dose, blood cell counts, and fatigue-like behavior measured as voluntary wheel-running activity. Next, we used oral minocycline treatments to reduce inflammation and found that minocycline reduces, but does not eliminate, the fatigue-like behavioral changes induced by radiation. We also used a strain of mice lacking the MyD88 adaptor protein and found that these mice also showed less fatigue-like behavior than the wild-type controls. Finally, using serum and brain tissue samples, we determined changes in inflammatory signaling induced by irradiation in wild-type, minocycline treated, and MyD88 knockout mice. We found that irradiation increased serum levels of IL-6, a change that was partially reversed in mice treated with minocycline or lacking MyD88. Overall, our results suggest that inflammation plays a causal role in radiation-induced fatigue and that IL-6 may be an important mediator. Keywords: CCL, chemokine (CC) ligand; CD30 L, CD30 ligand; CFS, chronic fatigue syndrome; CRF, cancer-related fatigue; CXCL, chemokine (CXC) ligand; Cancer-related fatigue; Cytokines; FGF, fibroblast growth factor; Fas-L, Fas Ligand; Fatigue; G-CSF, granulocyte colony-stimulating factor; GM-CSF, granulocyte-macrophage colony-stimulating factor; ICAM, intercellular adhesion molecule; IFN, interferon; IL, interleukin; Inflammation; LIF, leukemia inhibitory factor; M-CSF, macrophage colony-stimulating factor; MCV, mean corpuscular volume; Minocycline; MyD88, myeloid differentiation primary response 88 protein; PDGF-bb, platelet-derived growth factor subunit B; RANTES, regulated on activation normal T cell expressed and secreted; RBC, red blood cell; Radiotherapy; TIMP, tissue inhibitor of metalloproteinases; TLR, toll-like receptor; TNF, tumor necrosis factor; VEGF, vascular endothelial growth factor; VWRA, voluntary wheel running activity; Voluntary wheel-running activity; WBC, white blood cell; myd88.

---

 

乐备实是国内专注于提供高质量蛋白检测以及组学分析服务的实验服务专家，自2018年成立以来，乐备实不断寻求突破，公司的服务技术平台已扩展到单细胞测序、空间多组学、流式检测、超敏电化学发光、Luminex多因子检测、抗体芯片、PCR Array、ELISA、Elispot、PLA蛋白互作、多色免疫组化、DSP空间多组学等30多个，建立起了一套涵盖基因、蛋白、细胞以及组织水平实验的完整检测体系。

 
我们可提供从样本运输、储存管理、样本制备、样本检测到检测数据分析的全流程服务。凭借严格的实验室管理流程、标准化实验室操作、原始数据储存体系以及实验项目管理系统，已经为超过3000家客户单位提供服务，年检测样本超过100万，受到了广大客户的信任与支持。