Secondary lymphoid organs (SLOs), such as lymph nodes and tonsils, serve as an interface between the immune system and tumor cells as an initial antigen presentation site, critical in anti-tumor immune response and disease progression. For oropharyngeal cancers (OPCs) originating from palatine tonsils, we hypothesized that characterizing the immunological process occurring at the peri-tumoral tonsillar tissue will elucidate immune mechanisms for the lymphatic spread of the disease. A total of 33 patients were enrolled and subdivided into two cohorts. For cohort 1 (6 cases), gene expression profiles at the peri-tumoral lymphoid regions and tumor regions were analyzed using the GeoMx whole transcriptome atlas. In the peri-tumoral lymphoid regions, 237 genes were upregulated in metastasis-negative cases compared with metastasis-positive ones, but only one gene in tumor regions. For cohort 2 (27 cases), microarray analysis of peri-tumoral tonsillar tissues revealed 192 upregulated genes. Gene Ontology (GO) analyses revealed the significantly enriched GO terms associated with T cell activation and detected 10 hub genes according to the degree rank (PTPRC, TLR4, CD80, CD40, STAT3, CD28, CD40LG, CD44, CCR7, and IL7R). Gene set enrichment analysis combined with principal component analysis effectively sorted patients with or without lymph node metastases. These findings suggest peri-tumoral tonsils as a potential target to investigate the immune mechanisms associated with the lymphatic spread of the disease in OPCs.

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乐备实（上海优宁维生物科技股份有限公司旗下全资子公司），是国内专注于提供高质量蛋白检测以及组学分析服务的实验服务专家，自2018年成立以来，乐备实不断寻求突破，公司的服务技术平台已扩展到单细胞测序、空间多组学、流式检测、超敏电化学发光、Luminex多因子检测、抗体芯片、PCR Array、ELISA、Elispot、PLA蛋白互作、多色免疫组化、DSP空间多组学等30多个，建立起了一套涵盖基因、蛋白、细胞以及组织水平实验的完整检测体系。

 
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