Histamine receptor, one typical G-protein-coupled receptor (GPCR), can be activated by histamine and form the most important drug targets involved in allergy and reflux diseases. Here, we report an artificial model to mimic the wettability-induced activation of natural GPCRs via histamine-modulated enhancement of wettability in a bionic nanochannel. The artificial receptor is constructed by introducing key recognition factors in nature, L-lysine modified fluorescein isothiocyanate (L-lysine-FITC), into a conical nanochannel. The conductance of the L-lysine-FITC-modified nanochannel increases with the histamine-induced wettability enhancement due to the various interactions between histamine and L-lysine-FITC molecules including hydrogen bonding and π-π interactions, as well as the proton transfer reaction. This study represents a crucial step towards the design of artificial GPCRs with wettability-induced activation and provides an opportunity to construct artificial models of GPCRs in a non-lipid environment. The developed artificial receptor has great potential application in medicinal chemistry, biosensors, and healthcare systems.

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乐备实（上海优宁维生物科技股份有限公司旗下全资子公司），是国内专注于提供高质量蛋白检测以及组学分析服务的实验服务专家，自2018年成立以来，乐备实不断寻求突破，公司的服务技术平台已扩展到单细胞测序、空间多组学、流式检测、超敏电化学发光、Luminex多因子检测、抗体芯片、PCR Array、ELISA、Elispot、PLA蛋白互作、多色免疫组化、DSP空间多组学等30多个，建立起了一套涵盖基因、蛋白、细胞以及组织水平实验的完整检测体系。

 
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