Landscape of Infiltrating T Cells in Liver Cancer Revealed by Single-Cell Sequencing

cancer;癌症;肿瘤;单细胞测序;单细胞多组学

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Chunhong Zheng, Liangtao Zheng, Jae-Kwang Yoo, Huahu Guo, Yuanyuan Zhang, Xinyi Guo, Boxi Kang, Ruozhen Hu, Julie Y Huang, Qiming Zhang, Zhouzerui Liu, Minghui Dong, Xueda Hu, Wenjun Ouyang, Jirun Peng, Zemin Zhang

期刊 Cell
时间 2017
影响因子 66.85
起止号 169(7):1342-1356.e16.
种属 Human
技术平台单细胞测序
样本类型 T cells isolated from peripheral blood, tumor and adjacent normal tissues from hepatocellular carcinoma patients.
研究方向消化系统
细胞数 5063
细胞类型 T细胞
疾病类型肝癌
检测基因 ABLM1, ACTN1, AIF1, APBA2, APEX1, HMHA1, ARHGI, ATM, BACH2, BEX4, CA162, CA228, G8, CAH6, KCC4, CCR7, CD248, DAF, CEP68, COQ8A, DGKA, EF2, EIF3E, EIF3L, HYES, EXOS8, F102A, F117B, NA, FKBP5, FOXP1, NA, GCSAM, GIMA2, NA, GP155, GP183, IGF1R, ITFG2, LDHB, ARH, LEF1, NA, NA, LMO7, LR75A, C1AS1, LRRN3, MAL, MAML2, MLXIP, MYC, NFIP1, NELL2, NOGG, NOSIP, NPAT, NPM, 5NTD, OXND1, PASK, PDE3B, PDK1, P3IP1, PKHB1, NA, PSIP1, R3HD4, RACK1, GRP2, RCAN3, RIC3, RS8, SCML4, LYAM1, SERC5, SGSM3, SH3Y1, SMAP2, SNED1, SNURF, SPIT2, SPTB2, STMN3, TCF7, THAP7, THEM4, PORIM, TM204, CSCL1, TNFL8, TOM7, TPC1, TIKI1, TRAP1, TRM1, TXK, UBQL2, VIPR1, WDR43, ZN101
数据库ID GSE98638

Abstract

Systematic interrogation of tumor-infiltrating lymphocytes is key to the development of immunotherapies and the prediction of their clinical responses in cancers. Here, we perform deep single-cell RNA sequencing on 5,063 single T cells isolated from peripheral blood, tumor, and adjacent normal tissues from six hepatocellular carcinoma patients. The transcriptional profiles of these individual cells, coupled with assembled T cell receptor (TCR) sequences, enable us to identify 11 T cell subsets based on their molecular and functional properties and delineate their developmental trajectory. Specific subsets such as exhausted CD8+ T cells and Tregs are preferentially enriched and potentially clonally expanded in hepatocellular carcinoma (HCC), and we identified signature genes for each subset. One of the genes, layilin, is upregulated on activated CD8+ T cells and Tregs and represses the CD8+ T cell functions in vitro. This compendium of transcriptome data provides valuable insights and a rich resource for understanding the immune landscape in cancers.

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Abstract