Single-cell analysis reveals the continuum of human lympho-myeloid progenitor cells

single cell sequencing;SNS;单细胞测序;单细胞多组学

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Dimitris Karamitros, Bilyana Stoilova, Zahra Aboukhalil, Fiona Hamey, Andreas Reinisch, Marina Samitsch, Lynn Quek, Georg Otto, Emmanouela Repapi, Jessica Doondeea, Batchimeg Usukhbayar, Julien Calvo, Stephen Taylor, Nicolas Goardon, Emmanuelle Six, Francoise Pflumio, Catherine Porcher, Ravindra Majeti, Berthold Göttgens, Paresh Vyas

期刊 Nat Immunol
时间 2018
影响因子 31.25
起止号 19(1):85-97.
种属 Human
技术平台单细胞测序
样本类型 Single Cell RNA sequencing analysis of human haemopoietic lympho-myeloid progenitor populations (LMPP, MLP and GMP)
研究方向技术分享
细胞数 420
检测基因 ADA19, LX15B, ANR44, ARFG1, APRV1, B3GA2, NA, CT197, CACB4, CAPSL, CC168, CD34, CD47, CDK9, CLIP3, COBL, CO6A3, CACP, NA, CYTIP, DPYL3, COE1, EDC4, ETS1, F210B, FCRL3, FGD6, GAS7, GRM1B, GRB2, HERC4, NA, KCTD9, NA, KLHL6, KLKB1, NTAL, LCAT, LDB2, NA, NA, NA, LY65C, MMP28, MTUS1, MYO1G, NKAI2, NPL, OAS2, OXR1, PADI4, PDE1A, PLAL2, PLCX2, P2R3B, PRAME, PROB1, PROM1, PROX2, PSPN, PD2R2, NA, NADC, RN103, RN213, RNPL1, FTM, NA, S10A8, NA, NA, S22AH, SNX10, SRB4D, SIAT9, ST32B, STB5L, TARB1, NA, TRML1, TRPM2, UBP2, VPS54, TISD, ZKSC8, ZN367, ZSWM1
数据库ID GSE100618

Abstract

The hierarchy of human hemopoietic progenitor cells that produce lymphoid and granulocytic-monocytic (myeloid) lineages is unclear. Multiple progenitor populations produce lymphoid and myeloid cells, but they remain incompletely characterized. Here we demonstrated that lympho-myeloid progenitor populations in cord blood - lymphoid-primed multi-potential progenitors (LMPPs), granulocyte-macrophage progenitors (GMPs) and multi-lymphoid progenitors (MLPs) - were functionally and transcriptionally distinct and heterogeneous at the clonal level, with progenitors of many different functional potentials present. Although most progenitors had the potential to develop into only one mature cell type ('uni-lineage potential'), bi- and rarer multi-lineage progenitors were present among LMPPs, GMPs and MLPs. Those findings, coupled with single-cell expression analyses, suggest that a continuum of progenitors execute lymphoid and myeloid differentiation, rather than only uni-lineage progenitors' being present downstream of stem cells.

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Abstract