Multiplexed quantification of proteins and transcripts in single cells
single cell sequencing;SNS;单细胞测序;单细胞多组学浏览次数：22 分享： 
- Nat Biotechnol
- 2017
- 68.164
- 35(10):936-939.
- Human
- 单细胞测序
- DNA sequencing of both mRNA and antibodies in a single workflow in human PBMCs and na?ve CD8+ T cells that were costimulated with either 1) aCD3 and aCD28 or 2) aCD3, aCD28 and aCD27
- 技术分享
- 其它细胞
- GSE100501
Abstract
We present a tool to measure gene and protein expression levels in single cells with DNA-labeled antibodies and droplet microfluidics. Using the RNA expression and protein sequencing assay (REAP-seq), we quantified proteins with 82 barcoded antibodies and >20,000 genes in a single workflow. We used REAP-seq to assess the costimulatory effects of a CD27 agonist on human CD8+ lymphocytes and to identify and characterize an unknown cell type.
金课堂之文献解析 文献原文请点击
关注微信公众号
关注微信视频号
本网站销售的所有产品及服务均不得用于人类或动物之临床诊断或治疗，仅可用于工业或者科研等非医疗目的。
沪公网安备31011502400759号
营业执照(三证合一)
labex@univ-bio.com
上海市浦东新区古丹路15弄18号楼4楼
