G&T-seq: parallel sequencing of single-cell genomes and transcriptomes

single cell sequencing;SNS;单细胞测序;单细胞多组学

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Iain C Macaulay, Wilfried Haerty, Parveen Kumar, Yang I Li, Tim Xiaoming Hu, Mabel J Teng, Mubeen Goolam, Nathalie Saurat, Paul Coupland, Lesley M Shirley, Miriam Smith, Niels Van der Aa, Ruby Banerjee, Peter D Ellis, Michael A Quail, Harold P Swerdlow, Magdalena Zernicka-Goetz, Frederick J Livesey, Chris P Ponting, Thierry Voet

期刊 Nat Methods
时间 2015
影响因子 47.99
起止号 12(6):519-22.
种属 Human,Mouse
技术平台单细胞测序
样本类型 human breast cancer cells, lymphoblastoid cells. Mice ESCs
研究方向技术分享
细胞数 700
细胞类型肿瘤细胞
数据库ID EGAD00001001332

Abstract

The simultaneous sequencing of a single cell's genome and transcriptome offers a powerful means to dissect genetic variation and its effect on gene expression. Here we describe G&T-seq, a method for separating and sequencing genomic DNA and full-length mRNA from single cells. By applying G&T-seq to over 220 single cells from mice and humans, we discovered cellular properties that could not be inferred from DNA or RNA sequencing alone.

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Abstract