RNA binding protein HuD mediates the crosstalk between β cells and islet endothelial cells by the regulation of Endostatin and Serpin E1 expression

RNA binding protein HuD plays essential roles in gene expression by regulating RNA metabolism, and its dysregulation is involved in the pathogenesis of several diseases, including tumors, neurodegenerative diseases, and diabetes. Here, we explored HuD-mediated differential expression of secretory proteins in mouse insulinoma βTC6 cells using a cytokine array. Endostatin and Serpin E1 that play anti-angiogenic roles were identified as differentially expressed proteins by HuD. HuD knockdown increased the expression of α chain of collagen XVIII (Col18a1), a precursor form of endostatin, and Serpin E1 by associating with the 3'-untranslated regions (UTRs) of Col18a1 and Serpin E1 mRNAs. Reporter analysis revealed that HuD knockdown increased the translation of EGFP reporters containing 3'UTRs of Col18a1 and Serpin E1 mRNAs, which suggests the role of HuD as a translational repressor. Co-cultures of βTC6 cells and pancreatic islet endothelial MS1 cells were used to assess the crosstalk between β cells and islet endothelial cells, and the results showed that HuD downregulation in βTC6 cells inhibited the growth and migration of MS1 cells. Ectopic expression of HuD decreased Col18a1 and Serpin E1 expression, while increasing the markers of islet vascular cells in the pancreas of db/db mice. Taken together, these results suggest that HuD has the potential to regulate the crosstalk between β cells and islet endothelial cells by regulating Endostatin and Serpin E1 expression, thereby contributing to the maintenance of homeostasis in the islet microenvironment.

浏览次数：30 分享：

Myeongwoo Jung, Seungyeon Ryu, Chongtae Kim, Seongho Cha, Hoin Kang, Eunbyul Ji, Youlim Hong, Youngjoon Lee, Sukyoung Han, Seung Min Jeong, Wook Kim, Eun Kyung Lee

期刊 Cell Death Dis
影响因子 9
起止号 2022 Dec 5;13(12):1019.
种属 Mouse
技术平台抗体芯片
应用领域生物标志物
研究方向生物标志物
细胞类型内皮细胞
检测蛋白 Adiponectin/Acrp30,DPPIV/CD26,IL-27,Amphiregulin,EGF,IL-28,Angiopoietin-1,Endoglin/CD105,IL-33,Angiopoietin-2,Endostatin,LDL R,Angiopoietin-like 3,Fetuin A/AHSG,Leptin,BAFF/BLyS/TNFSF13B,FGF acidic,LIF,C1q R1/CD93,FGF-21,Lipocalin-2/NGAL,CCL2/JE/MCP-1,Flt-3 Ligand,LIX,CCL3/CCL4 MIP-1 alpha/beta,Gas6,M-CSF,CCL5/RANTES,G-CSF,MMP-2,CCL6/C10,GDF-15,MMP-3,CCL11/Eotaxin,GM-CSF,MMP-9,CCL12/MCP-5,HGF,Myeloperoxidase,CCL17/TARC,ICAM-1/CD54,Osteopontin (OPN),CCL19/MIP-3 beta,IFN-gamma,Osteoprotegerin/TNFRSF11B,CCL20/MIP-3 alpha,IGFBP-1,PD-ECGF/Thymidine phosphorylase,CCL21/6Ckine,IGFBP-2,PDGF-BB,CCL22/MDC,IGFBP-3,Pentraxin 2/SAP,CD14,IGFBP-5,Pentraxin 3/ TSG-14,CD40/TNFRSF5,IGFBP-6,Periostin/OSF-2,CD160,IL-1 alpha/IL1F1,Pref-1/DLK-1/FA1,Chemerin,IL-1 beta/IL-1F2,Proliferin,Chitinase 3-like 1,IL-1ra/IL-1F3,Proprotein Convertase 9/PCSK9,Coagulation Factor III/Tissue Factor,IL-2,RAGE,Complement Component C5/C5a,IL-3,RBP4,Complement Factor D,IL-4,Reg3G,C-Reactive Protein/CRP,IL-5,Resistin,CX3CL1/Fractalkine,IL-6,E-Selectin/CD62E,CXCL1/KC,IL-7,P-Selectin/CD62P,CXCL2/MIP-2,IL-10,Serpin E1/PAI-1,CXCL9/MIG,IL-11,Serpin F1/PEDF,CXCL10/IP-10,IL-12p40,Thrombopoietin,CXCL11/I-TAC,IL-13,TIM-1/KIM-1/HAVCR,CXCL13/BLC/BCA-1,IL-15,TNF-alpha,CXCL16,IL-17A,VCAM-1/CD106,Cystatin C,IL-22,VEGF,Dkk-1,IL-23,WISP-1/CCN4

Abstract

RNA binding protein HuD plays essential roles in gene expression by regulating RNA metabolism, and its dysregulation is involved in the pathogenesis of several diseases, including tumors, neurodegenerative diseases, and diabetes. Here, we explored HuD-mediated differential expression of secretory proteins in mouse insulinoma βTC6 cells using a cytokine array. Endostatin and Serpin E1 that play anti-angiogenic roles were identified as differentially expressed proteins by HuD. HuD knockdown increased the expression of α chain of collagen XVIII (Col18a1), a precursor form of endostatin, and Serpin E1 by associating with the 3'-untranslated regions (UTRs) of Col18a1 and Serpin E1 mRNAs. Reporter analysis revealed that HuD knockdown increased the translation of EGFP reporters containing 3'UTRs of Col18a1 and Serpin E1 mRNAs, which suggests the role of HuD as a translational repressor. Co-cultures of βTC6 cells and pancreatic islet endothelial MS1 cells were used to assess the crosstalk between β cells and islet endothelial cells, and the results showed that HuD downregulation in βTC6 cells inhibited the growth and migration of MS1 cells. Ectopic expression of HuD decreased Col18a1 and Serpin E1 expression, while increasing the markers of islet vascular cells in the pancreas of db/db mice. Taken together, these results suggest that HuD has the potential to regulate the crosstalk between β cells and islet endothelial cells by regulating Endostatin and Serpin E1 expression, thereby contributing to the maintenance of homeostasis in the islet microenvironment.

首页 >

技术资源 >

文献中心 >

相关货号

Abstract